The ISTARI Care Cube is a novel isolation unit for highly infectious and emerging pathogens. This unit flips the traditional approach to personal protective equipment by isolating the patient, thereby promoting the safety of healthcare workers and other patients. The University of Washington, in partnership with the University of Nebraska, has been evaluating the safety and usability of various models. This video shows an early version of the 1c model being assessed for use in pre-hospital transport. This work is made possible by a CDC National Infection Control Strengthening associated with its Project Firstline.